Spheroids - FAQ´s
Due to the great interest in our
spheroid based culture techniques we have put together frequently asked
questions (FAQ´s) in a short catalogue. We hope that this catalogue
can adress most of your technical questions and provide useful hints to
bring the spheroids in your lab. If you feel that some other relevant questions
should be adressed or if you have further problems to establish the spheroids
in your lab do not hestitate to contact us.
the spheroid culture technique:
the spheroid based sprouting assay:
Which cell culture plastic do you use
to generate spheroids?
Which cell types have been tested for
their spheroid forming capacity?
How do you prepare the methocel stock
solution which is needed for spheroid culture?
How do you generate the shperoids containig
a precise number of cells?
How long can you culture the spheroids?
How do you harvest the spheroids?
How fragile are the spheroids?
paraffin embedding of spheroids:
How do you prepare the collagen stock
solution needed for the spheroid based angiogenesis assay?
How do you prepare the collagen gels
for the spheroid based angiogenesis assay?
How can I decrease baseline sprouting
in spheroid based angiogenesis assays?
How long let you run the spheroid based
How can spheroids be embedded into paraffin?
Is it possible to embed collagen gels
in paraffin to analyze sprouts originating from the spheroids?