Due to the great interest in our spheroid based culture techniques we have put together frequently asked questions (FAQ´s) in a short catalogue. We hope that this catalogue can adress most of your technical questions and provide useful hints to bring the spheroids in your lab. If you feel that some other relevant questions should be adressed or if you have further problems to establish the spheroids in your lab do not hestitate to contact us.

Questions regarding the spheroid culture technique:

• Which cell culture plastic do you use to generate spheroids?
• Which cell types have been tested for their spheroid forming capacity?
• How do you prepare the methocel stock solution which is needed for spheroid culture?
• How do you generate the shperoids containig a precise number of cells?
• How long can you culture the spheroids?
• How do you harvest the spheroids?
• How fragile are the spheroids?

• How do you prepare the collagen stock solution needed for the spheroid based angiogenesis assay?
• How do you prepare the collagen gels for the spheroid based angiogenesis assay?
• How can I decrease baseline sprouting in spheroid based angiogenesis assays?
• How long let you run the spheroid based sprouting assay?

• How can spheroids be embedded into paraffin?
• Is it possible to embed collagen gels in paraffin to analyze sprouts originating from the spheroids?